General description

This protein is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

Product Source: N-terminal FLAG-tagged, recombinant, human LRRK2 amino acids 970-end, expressed by baculovirus in Sf21 insect cells.

Legal Information

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Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250µg size as catalog # 14-919M and in bulk as catalog # 14-919-K.

Physical form

50mM Tris/HCl pH7.4, 300mM NaCl, 20mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 150µg/ml FLAG peptide, 20% v/v glycerol, 1mM benzamidine, 0.2mM PMSF, 5mM DTT. Frozen solution.

Quality

Routinely evaluated by phosphorylation of ERMtide (RLGRDKYKTLRQIRQ)